Focus on the low rooting rate, few lateral roots, brittle texture and low survival rate of transplanting peach tissue culture seedlings, the sugar-free roting technology was used in this study. With the tissue culture seedlings of Amygdalus davidiana as experimental materials, the effects of auxin and vermiculite water content on the root development of peach were studied. The growth and development of root system and aboveground part of peach in different periods were observed. The results showed that :①The rooting condition was the best in the case that the auxin was 1.0 mg/L IBA. ②The suitable vermiculite water content of sugar-free rooting was 65%~70%. ③On the 10th day of tissue culture, obvious root tips could be observed. The rooting rate reached 100% on the 25th day, and the 35th day was the most suitable for transplantation. Rooting without sugar could significantly improve the rooting condition of tissue culture seedlings of peach, the rooting rate and average number of roots were significantly increased, the seedlings gew healthily, the root system was developed, the operation was simple, which was suitable for popularization and application in production. |