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山桃组培苗无糖生根培养研究
Study on Sugar-free Rooting Culture in Tissue Culture Plantlets of Prunus davidiana
  
DOI:
中文关键词:  山桃  组培苗  无糖生根  根系扫描
英文关键词:Prunus davidiana   Tissue culture seedling  Sugar-free rooting  Root scanning
基金项目:陕西省重点研发计划(2018NY-048)。
作者单位
刘福云,孙怡婷,徐一超,卢 显,张 东,赵彩平 (西北农林科技大学 园艺学院, 陕西 杨凌 712100) 
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中文摘要:
      针对桃组培苗生根率低,侧根少,质地脆,移栽成活率低的情况,研究采用无糖生根技术,以山桃(Prunus davidiana)组培苗为实验材料,探讨了生长素、蛭石含水量对山桃根系发育的影响,并观察了不同时期山桃根系和地上部的生长发育情况。结果表明:①生长素为1.0 mg/L IBA时,山桃生根情况最好。②适宜山桃无糖生根的蛭石含水量为65%~70%。③山桃组培苗培养到第10天可以观察到明显的根尖,第25天时生根率达到100%,第35天时最适合移栽。无糖生根能明显改善山桃组培苗的生根情况,生根率和平均生根数显著提高,苗子生长健壮,根系发达,操作简单,适宜在生产中推广使用。
英文摘要:
      Focus on the low rooting rate, few lateral roots, brittle texture and low survival rate of transplanting peach tissue culture seedlings, the sugar-free roting technology was used in this study. With the tissue culture seedlings of Amygdalus davidiana as experimental materials, the effects of auxin and vermiculite water content on the root development of peach were studied. The growth and development of root system and aboveground part of peach in different periods were observed. The results showed that :①The rooting condition was the best in the case that the auxin was 1.0 mg/L IBA. ②The suitable vermiculite water content of sugar-free rooting was 65%~70%. ③On the 10th day of tissue culture, obvious root tips could be observed. The rooting rate reached 100% on the 25th day, and the 35th day was the most suitable for transplantation. Rooting without sugar could significantly improve the rooting condition of tissue culture seedlings of peach, the rooting rate and average number of roots were significantly increased, the seedlings gew healthily, the root system was developed, the operation was simple, which was suitable for popularization and application in production.
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